Surge Scientific · Model FLR200HS · High-Sensitivity Fluorometer · DNA / RNA / Protein
Fluorometer · FLR200HS
2.5 Sec/Sample · <10 pg/µL dsDNA · 1–20 µL Sample · 10,000 Programs
Blue LED (470 nm) + Red LED (635 nm) · Lower Limit of Detection <10 pg/µL dsDNA · DNA · RNA · Protein · NGS Library QC · Western Blot · Touchscreen · USB Export
The FLR200HS is the high-sensitivity fluorometer for nucleic acid and protein quantification when your sample is too precious to waste on a spectrophotometer. Lower limit of detection <10 pg/µL for dsDNA, 1–20 µL sample volume, and a 2.5-second read per sample deliver fluorescence-based quantification that's 1000× more sensitive than absorbance methods and specific to the target analyte — DNA, RNA, or protein — instead of measuring everything that absorbs at 260 nm. Touchscreen interface, 10,000-program data storage, USB export, and dual blue (470 nm) + red (635 nm) excitation LEDs covering the standard fluorescent dye assay menu.
Why Fluorometry Beats UV Spectrophotometry for NGS & Low-Concentration Samples
UV spectrophotometers like NanoDrop measure absorbance at 260 nm — but at 260 nm, everything absorbs: DNA, RNA, free nucleotides, residual proteins, salts, phenol, EDTA. The instrument can't distinguish between actual amplifiable DNA and residual contamination, which is why NanoDrop measurements often overestimate concentration by 3–4× compared to fluorometric assays. For routine QC, that's tolerable. For NGS library prep, where 10× concentration error means a sequencing run worth thousands of dollars produces unbalanced libraries, it's not.
Fluorometry uses analyte-specific dyes — Picogreen-class dyes for dsDNA, RiboGreen for RNA, dedicated dyes for ssDNA and protein. The dye binds only to the target molecule, then fluoresces when excited. Free nucleotides, residual protein, salts — none of them produce signal. The result is a measurement that's 1000× more sensitive than absorbance (down to <10 pg/µL for the FLR200HS) and specific to the analyte you actually care about. For NGS library QC, ctDNA quantification, low-abundance RNA, and any sample where contaminants might be present, fluorometry isn't optional — it's the right answer.
<10 pg/µL dsDNA Sensitivity
Lower limit of detection <10 pg/µL for dsDNA — femtogram-class quantification for ctDNA, NGS libraries, low-yield extractions, and degraded samples.
2.5 Sec/Sample · 1–20 µL
2.5-second read per sample, 1–20 µL sample volume — minimum sample consumed, maximum throughput for QC pipelines.
Dual LED · Multi-Analyte
Blue LED (470 nm peak) + Red LED (635 nm peak) excitation covers the standard fluorescent dye menu — dsDNA, ssDNA, RNA, and protein.
10,000 Programs · Touchscreen
Touchscreen interface, 10,000 program data storage, USB export. Dedicated assay protocols for dsDNA, RNA, protein — and user-defined dye protocols for custom workflows.
"<10 pg/µL detection limit. 2.5 seconds per sample. The fluorometer that makes NGS library QC, ctDNA, and low-abundance work actually quantifiable."
Key Features
- High-sensitivity fluorometer
- Lower limit of detection <10 pg/µL dsDNA
- 2.5-second read time per sample
- 1–20 µL sample volume
- Blue LED excitation (470 nm peak)
- Red LED excitation (635 nm peak)
- dsDNA quantification
- ssDNA quantification
- RNA quantification
- Protein quantification
- NGS library prep QC
- Western blot protein analysis
- Pre-programmed assay protocols
- User-defined dye protocols
- Compatible with standard fluorescent dye assays
- Touchscreen interface
- Intuitive operation
- Stand-alone — no PC required
- 10,000 program data storage
- USB drive data export
- Compact bench footprint
- Compatible with PCR tube format
- Single-point and standard curve calibration
- Suitable for research labs
- Suitable for clinical diagnostic labs
- Suitable for NGS facilities
- 2-year all-inclusive warranty
Ideal Applications
NGS Library QC
Library quantification before sequencer loading — fluorometric measurement gives you the amplifiable molecule count (not the contamination-inflated absorbance number).
cfDNA / ctDNA Quantification
Cell-free DNA from liquid biopsy and circulating tumor DNA — sub-10 pg/µL sensitivity reaches the concentration ranges that liquid biopsy actually requires.
Protein & RNA Workflows
Protein quantification for Western blot prep, RNA quantification for RT-PCR / RNA-seq normalization — analyte-specific accuracy where absorbance contamination matters.
What's in the Box
FLR200HS ships ready for fluorometric quantification — touchscreen, dual LED system, USB ready. Reagent kits sold separately.
Technical Specifications
| Parameter | Specification |
|---|---|
| Model / SKU | FLR200HS |
| Light Sources | Blue LED (470 nm peak) + Red LED (635 nm peak) |
| Lower Limit of Detection (dsDNA) | <10 pg/µL |
| Read Time | 2.5 seconds per sample |
| Sample Volume Range | 1–20 µL |
| Analyte Compatibility | dsDNA · ssDNA · RNA · Protein |
| Pre-Programmed Assays | Multiple — dsDNA, RNA, protein, etc. |
| User-Defined Protocols | Yes |
| Display | Touchscreen |
| Operation Mode | Stand-alone — no PC required |
| Data Storage | 10,000 programs |
| Data Export | USB drive |
| Calibration | Single-point + standard curve |
| Tube Compatibility | Standard PCR tubes |
| Applications | NGS library QC · cfDNA · ctDNA · RNA QC · Western blot prep |
| Warranty | 2-year all-inclusive |
Surge Scientific Warranty & Support
This instrument is sold through Surge Scientific, your trusted laboratory equipment partner. Our product specialists are available Monday–Friday for assistance. The FLR200HS is backed by a 2-year all-inclusive manufacturer's warranty. Contact us at info@surgescientific.com or reach out directly to our support team at 855.943.3344 for pre-sales questions, reagent kit selection, NGS QC workflow, calibration protocols, and technical assistance.