Surge Scientific · Tianlong Gentier 96E · IVDR CE Certified
Gentier 96E Real-Time PCR System
96 Wells · 6 Fluorescence Channels · 7-Second Full-Plate Scan · ±0.1°C Uniformity · 10.4" Touch Screen
Most 96-well qPCR platforms make you choose between throughput and optical precision — slower scanning means more stable readings, or so the assumption goes. The Gentier 96E disproves that trade-off: all 96 wells across all 6 fluorescence channels are scanned in 7 seconds, while ±0.1°C block uniformity and Peltier-driven ramp rates above 6.1°C/s ensure that speed never comes at the expense of thermal accuracy.
7s
Full 96-Well Scan
6
Fluorescence Channels
>6.1°C/s
Max Heating Rate
±0.1°C
Block Uniformity
Why Speed Without Uniformity is Useless in qPCR
Fluorescence scanning speed and thermal block performance are the two parameters most labs examine separately — but they fail or succeed together. A fast scanner on a thermally uneven block amplifies the unevenness: the wells that heat faster reach denaturation first, those that cool slower anneal late, and what looks like biological variation in your Ct values is actually instrument artifact. This is the hidden source of CV inflation in multi-well quantification runs, and it becomes especially damaging in multiplex panels where channel cross-talk compounds block non-uniformity.
The Gentier 96E addresses both vectors simultaneously. The Peltier-based thermal block delivers ±0.1°C uniformity across all 96 wells — not edge-compensated, not software-corrected, but physically uniform — while the top-scanning LED/photodiode optical system completes a full-plate fluorescence read in 7 seconds without edge effects. The result is a Ct CV of ≤0.5% and a linear correlation coefficient of |r| ≥ 0.999, across a 10-log dynamic range from 10¹ to 10¹⁰ copies.
7-Second Full-Plate Optical Scan
Simultaneous well-by-well scanning of all fluorescence channels completes in 7 seconds for all 96 wells — eliminating edge effects from sequential scanning and reducing total run time without sacrificing signal integrity. LED excitation from the top ensures consistent optical path geometry across every well position.
Peltier Thermal Block Precision
A maximum heating ramp rate of >6.1°C/s and cooling rate of >5.0°C/s with ±0.1°C block uniformity and ≤0.1°C accuracy. Supports 12-column gradient PCR (1°C–40°C range), Touch Down PCR, and long PCR protocols — all from a single instrument without hardware swaps.
Dual-Mode Standalone Operation
A 10.4-inch full-color touchscreen enables complete instrument control without a connected PC — run protocols, review results, and manage up to 1,000 stored experiments independently. Power-failure protection automatically resumes interrupted runs when power is restored, with no software intervention required.
Broad Dye Compatibility Across 6 Channels
Six fluorescence channels covering FAM/SYBR Green through FRET accommodate virtually every probe chemistry in current clinical and research use — including HEX/VIC, ROX, Cy5, and Alexa Fluor 680. Channel configurations match Bio-Rad CFX96 and Agilent Mx3005P layouts for seamless assay transfer.
Ct CV ≤ 0.5% · Linear correlation |r| ≥ 0.999 · 10-log dynamic range. These are not marketing claims — they are the repeatability and linearity thresholds required for IVDR CE certification and validated across the Gentier 96E's full 10¹ to 10¹⁰ copy dynamic range, using Ct value coefficient of variation as the primary acceptance criterion.
Key Features
- 96-well throughput — 1 to 96 samples per run
- Full-plate fluorescence scan in 7 seconds flat
- 6 fluorescence detection channels
- LED light source — high-brightness, maintenance-free
- Photodiode (PD) detector, top-scanning architecture
- No edge effects — simultaneous well-by-well detection
- Peltier heating/cooling — >6.1°C/s heating, >5.0°C/s cooling
- Block uniformity: ±0.1°C across all 96 wells
- Temperature accuracy: ≤0.1°C
- Gradient PCR: 12-column, 1°C–40°C range
- Touch Down PCR and Long PCR protocol support
- 10.4-inch full-color touchscreen — standalone operation
- PC software control via direct USB connection
- Power-failure auto-recovery — no data loss
- Stores up to 1,000 experimental results on-board
- USB data upload/download — no network required
- Absolute & relative quantification analysis
- SNP genotyping analysis
- Melting curve / dissociation curve analysis
- Customizable experiment report templates
- Compatible: 0.2mL 96-well plates, 8-tube strips, single tubes
- Fluorescence dynamic range: adjustable gain
- Sample dynamic range: 10¹ to 10¹⁰ copies
- IVDR CE certified · EU RoHS compliant · NMPA registered
6-Channel Fluorescence Configuration
The Gentier 96E's optical system covers the full range of probe chemistries used in clinical diagnostics, infectious disease testing, oncology panels, and molecular research — including both hydrolysis probe and intercalating dye formats.
Channel 1 · 465nm / 510nm
FAM / SYBR Green I
FAM · SYBR Green I · SYTO 9 · Eva Green · LC Green
Channel 2 · 527nm / 563nm
HEX / VIC
HEX · VIC · TET · JOE
Channel 3 · 580nm / 616nm
ROX / Texas Red
ROX · Texas Red
Channel 4 · 632nm / 664nm
Cy5
Cy5
Channel 5 · 680nm / 730nm
Alexa Fluor 680
Alexa Fluor 680
Channel 6 · 465nm / 616nm
FRET
FRET probes
Ideal Applications
Clinical Diagnostics
Infectious pathogen detection (viral, bacterial, mycoplasma, chlamydia), blood screening, viral hepatitis quantification, STI panels, and oncology biomarker testing in hospital and clinical reference labs.
Molecular Research
Absolute and relative gene expression quantification, SNP genotyping, melting curve analysis, and high-throughput screening in genomics, immunology, forensics, and pharmacogenomics research workflows.
Food, Vet & Environmental
Food pathogen and GMO detection, animal disease surveillance (avian influenza, ASF, FMD), environmental health monitoring, and agricultural pathogen screening at CDC and inspection bureau laboratories.
Connectivity & Compatibility
The Gentier 96E operates as a fully standalone instrument or under PC software control — with data transfer, consumable flexibility, and software compatibility designed for integration into existing lab workflows without infrastructure changes.
Technical Specifications
| Parameter | Specification |
|---|---|
| Sample & Throughput | |
| Model | Gentier 96E |
| Sample Throughput | 1–96 wells per run |
| Suitable Consumables | 0.2mL 96-well plates · 8-tube strips · single tubes (clear, frosted, white) |
| Sample Dynamic Range | 10¹ to 10¹⁰ copies |
| Linearity (|r|) | ≥ 0.999 |
| Repeatability (Ct CV) | ≤ 0.5% |
| Optical System | |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7 seconds (all 96 wells, all channels) |
| Detection Method | Simultaneous well-by-well scanning — no edge effects |
| Light Source | High-brightness, long-life, maintenance-free LED · top excitation |
| Detector | Photodiodes (PDs) · top scanning |
| Fluorescence Dynamic Range | Adjustable |
| Excitation Wavelengths | CH1: 465nm · CH2: 527nm · CH3: 580nm · CH4: 632nm · CH5: 680nm · CH6: 465nm |
| Detection Wavelengths | CH1: 510nm · CH2: 563nm · CH3: 616nm · CH4: 664nm · CH5: 730nm · CH6: 616nm |
| Dye Compatibility | CH1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green · CH2: HEX, VIC, TET, JOE · CH3: ROX, Texas Red · CH4: Cy5 · CH5: Alexa Fluor 680 · CH6: FRET |
| Thermal Block | |
| Heating Method | Peltier |
| Heating Rate | > 6.1°C/s (maximum) |
| Cooling Rate | > 5.0°C/s (maximum) |
| Temperature Uniformity | ±0.1°C |
| Temperature Accuracy | ≤ 0.1°C |
| Gradient Range | 1°C – 40°C |
| Gradient Block | 12 columns |
| Special PCR Protocols | Gradient PCR · Touch Down PCR · Long PCR |
| Software & Control | |
| Control Mode 1 | 10.4-inch full-color touch screen (standalone) |
| Control Mode 2 | PC direct control via software |
| PC Operating System | Windows 7 / 10 / 11 |
| Data Storage | Up to 1,000 experimental results stored on-board |
| Data Transfer | USB disk upload/download |
| Power Failure Protection | Automatic experiment recovery on power restore — no PC required |
| Reporting | Customizable experiment report templates |
| Analysis Functions | Absolute quantification · Relative quantification · SNP analysis · Melting curve analysis |
| Physical & Electrical | |
| Dimensions (W×L×H) | 555 × 475 × 484 mm |
| Weight (Net) | 30 kg |
| Power Supply | AC 100–240V, 50–60Hz · 900 VA |
| Certifications | IVDR CE · NMPA · EU RoHS |
Surge Scientific Warranty & Support
The Gentier 96E is sold through Surge Scientific, your trusted laboratory instrument partner. Our product specialists are available Monday–Friday for pre-sales questions, assay compatibility guidance, and technical support. This instrument is backed by a 2-year all-inclusive manufacturer's warranty. Contact us at info@surgescientific.com or call 855.943.3344 for quotes, demo requests, and application support.