Gentier 96R 4 Channel Real-Time PCR System

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Surge Scientific · Tianlong Gentier 96R · IVDR CE Certified

Gentier 96R Real-Time PCR System

96 Wells · 4 Fluorescence Channels · 7-Second Full-Plate Scan · ±0.1°C Uniformity · Standalone Touchscreen

The majority of clinical and research qPCR assays run on four dye channels — FAM, HEX/VIC, ROX, and Cy5. The Gentier 96R is built precisely around that reality: four optimized fluorescence channels covering every major probe chemistry in current diagnostic and research use, paired with the same Peltier thermal block and 7-second full-plate scanning engine that drives the 96E — at a configuration matched to how most labs actually run their panels.

7s

Full 96-Well Scan

4

Fluorescence Channels

>6.1°C/s

Max Heating Rate

±0.1°C

Block Uniformity

🔁

Drop-in compatible with Bio-Rad CFX96 assay panels. The Gentier 96R's 4-channel optical configuration — FAM, HEX/VIC, ROX, Texas Red, Cy5 — mirrors the CFX96 channel layout. Assays validated on the CFX96 transfer to the 96R without re-optimization, making it the lowest-friction upgrade path for labs standardizing on a cost-efficient high-throughput platform.

Why Four Channels Cover the Full Diagnostic Spectrum

The diagnostic PCR market runs predominantly on four dye channels. FAM anchors the primary target in virtually every clinical assay — from SARS-CoV-2 to HPV to MRSA. The second channel (HEX/VIC) carries the internal control in singleplex formats or the second target in duplex panels. ROX serves as the passive reference in most thermocycler platforms, and Cy5 handles the fourth target in multiplex infectious disease panels or the second probe in SNP genotyping. Labs that pay for a sixth channel and use it for two runs per year are over-instrumenting for a workflow that doesn't require it.

The Gentier 96R delivers the same Peltier thermal block, the same 7-second LED/photodiode scan architecture, and the same ±0.1°C uniformity as the 96E — optimized to four channels that cover 95% of published clinical qPCR assay formats. The result is full-platform performance tuned precisely to the workload of clinical diagnostics, infectious disease testing, and standard molecular research panels.

7-Second Full-Plate Scan, No Edge Effects

The top-scanning LED/photodiode optical system scans all 96 wells across all 4 channels in 7 seconds — simultaneous well-by-well detection eliminates the position-dependent signal variation that plagues sequential scanning systems. Every well in every row sees the same optical geometry, at the same moment in the thermal cycle.

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Peltier Block — Precision at Scale

A maximum heating rate of >6.1°C/s and cooling rate of >5.0°C/s keep thermal cycling tight across all 96 wells simultaneously. ±0.1°C block uniformity and ≤0.1°C temperature accuracy translate directly to Ct CV ≤0.5% — the repeatability benchmark required for validated clinical assay performance.

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Standalone Operation Without PC Dependency

A 10.4-inch full-color touchscreen runs protocols, stores up to 1,000 results, and manages all instrument functions independently. Power-failure protection resumes interrupted experiments automatically — no PC reboot, no software restart, no lost run — making the 96R reliable in environments where IT infrastructure is inconsistent.

📊

Complete Analysis Suite, On-Board

Absolute quantification, relative quantification, SNP genotyping, and melting curve analysis are all available directly on the instrument — no external software license required for routine workflows. Customizable report templates output publication-ready or regulatory-submission-ready data in a single export step.

|r| ≥ 0.999 linear correlation · Ct CV ≤ 0.5% · 10¹ to 10¹⁰ copy dynamic range. The Gentier 96R's validated performance parameters satisfy the quantification accuracy and repeatability requirements of IVDR CE certification — the same regulatory standard applied to in vitro diagnostic instruments in the European market.

Key Features

  • 96-well throughput — 1 to 96 samples per run
  • Complete 96-well fluorescence scan in 7 seconds
  • 4 optimized fluorescence detection channels
  • High-brightness, maintenance-free LED light source
  • Photodiode (PD) detector — top-scanning architecture
  • Simultaneous well-by-well detection — zero edge effects
  • Peltier heating: >6.1°C/s · Cooling: >5.0°C/s
  • Block uniformity: ±0.1°C across all 96 wells
  • Temperature accuracy: ≤0.1°C
  • Gradient PCR: 12 columns, 1°C–40°C range
  • Touch Down PCR and Long PCR protocol support
  • 10.4-inch full-color touchscreen — complete standalone operation
  • PC software control via direct USB connection
  • Power-failure auto-recovery — no data loss, no intervention
  • 1,000 experimental results stored on-board
  • USB data upload and download
  • Absolute and relative quantification
  • SNP genotyping analysis
  • Melting curve / dissociation curve analysis
  • Customizable report templates — export-ready output
  • Compatible: 0.2mL 96-well plates, 8-tube strips, single tubes
  • Sample dynamic range: 10¹ to 10¹⁰ copies
  • Ct CV ≤ 0.5% · Linear correlation |r| ≥ 0.999
  • IVDR CE certified · EU RoHS compliant · NMPA registered

4-Channel Fluorescence Configuration

The Gentier 96R's four channels cover every probe chemistry used in standard clinical diagnostics, infectious disease multiplex panels, SNP genotyping, and quantitative gene expression analysis — including both hydrolysis probe and intercalating dye formats.

CH 1 · 465nm / 510nm

FAM / SYBR Green I

FAM · SYBR Green I · SYTO 9 · Eva Green · LC Green

CH 2 · 527nm / 563nm

HEX / VIC

HEX · VIC · TET · JOE

CH 3 · 580nm / 616nm

ROX / Texas Red

ROX · Texas Red

CH 4 · 632nm / 664nm

Cy5

Cy5

Ideal Applications

🏥

Clinical Diagnostics

Infectious pathogen detection, blood screening, viral load quantification, STI panels, and oncology marker testing in hospital, clinical reference, and public health laboratories where validated 4-dye assay formats are the standard.

🧬

Molecular Research

Absolute and relative gene expression, SNP genotyping, melting curve analysis, and high-throughput quantitative PCR screening in genomics, immunology, forensic science, and pharmacogenomics research workflows.

🌿

Food, Vet & Environmental

Pathogen detection in food and dairy, animal disease surveillance, environmental health monitoring, and agricultural safety testing at CDC, inspection bureaus, and veterinary diagnostic centers running standardized 4-channel assay panels.

Connectivity & Compatibility

The Gentier 96R integrates into existing laboratory infrastructure without workflow disruption — running standalone via touchscreen or connected to PC software, with consumable compatibility spanning all standard 96-well formats.

10.4" Touch Screen PC Software Control USB Data Transfer 1,000 Results On-Board Win 7 / 10 / 11 96-Well Plates 8-Tube Strips Single Tubes (Clear / White) IVDR CE Certified EU RoHS Compliant NMPA Registered AC 100–240V Universal

Technical Specifications

Parameter Specification
Sample & Throughput
Model Gentier 96R
Sample Throughput 1–96 wells per run
Suitable Consumables 0.2mL 96-well plates · 8-tube strips · single tubes (clear, frosted, white)
Sample Dynamic Range 10¹ to 10¹⁰ copies
Linearity (|r|) ≥ 0.999
Repeatability (Ct CV) ≤ 0.5%
Optical System
Fluorescence Channels 4
Fluorescence Scanning Time 7 seconds (all 96 wells, all channels)
Detection Method Simultaneous well-by-well scanning — no edge effects
Light Source High-brightness, long-life, maintenance-free LED · top excitation
Detector Photodiodes (PDs) · top scanning
Fluorescence Dynamic Range Adjustable
Excitation Wavelengths CH1: 465nm · CH2: 527nm · CH3: 580nm · CH4: 632nm
Detection Wavelengths CH1: 510nm · CH2: 563nm · CH3: 616nm · CH4: 664nm
Dye Compatibility CH1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green · CH2: HEX, VIC, TET, JOE · CH3: ROX, Texas Red · CH4: Cy5
Thermal Block
Heating Method Peltier
Heating Rate > 6.1°C/s (maximum)
Cooling Rate > 5.0°C/s (maximum)
Temperature Uniformity ±0.1°C
Temperature Accuracy ≤ 0.1°C
Gradient Range 1°C – 40°C
Gradient Block 12 columns
Special PCR Protocols Gradient PCR · Touch Down PCR · Long PCR
Software & Control
Control Mode 1 10.4-inch full-color touch screen (standalone)
Control Mode 2 PC direct control via software
PC Operating System Windows 7 / 10 / 11
Data Storage Up to 1,000 experimental results stored on-board
Data Transfer USB disk upload/download
Power Failure Protection Automatic experiment recovery on power restore — no PC required
Reporting Customizable experiment report templates
Analysis Functions Absolute quantification · Relative quantification · SNP analysis · Melting curve analysis
Physical & Electrical
Dimensions (W×L×H) 555 × 475 × 484 mm
Weight (Net) 30 kg
Power Supply AC 100–240V, 50–60Hz · 900 VA
Certifications IVDR CE · NMPA · EU RoHS
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Surge Scientific Warranty & Support

The Gentier 96R is sold through Surge Scientific, your trusted laboratory instrument partner. Our product specialists are available Monday–Friday for pre-sales questions, assay compatibility guidance, and technical support. This instrument is backed by a 2-year all-inclusive manufacturer's warranty. Contact us at info@surgescientific.com or call 855.943.3344 for quotes, demo requests, and application support.