Surge Scientific · CF321 · High-Speed Refrigerated Centrifuge
High-Speed Refrigerated Centrifuge — 15,000 RPM · 21,300 × g · −10°C to +40°C
Nucleic Acid Extraction · Protein Isolation · Cell Harvesting · Spin Columns · PCR Prep · 24 × 1.5/2.0 mL Standard Rotor Included
Temperature-sensitive samples degrade the moment centrifugation ends in an unrefrigerated instrument — and there is no recovering that run. The CF321 keeps the rotor chamber at your set temperature from pre-cool through the final second of deceleration, pairing 21,300 × g of separation force with precision cold-control that reaches 4°C in under five minutes from room temperature.
Why Refrigerated Separation Changes the Result
Standard high-speed microcentrifuges generate significant frictional heat at 15,000 RPM — enough to degrade labile enzymes, depolymerize thermolabile proteins, and introduce variability into downstream qPCR and NGS workflows. Researchers compensate by chilling tubes on ice before and after each spin, accepting reduced throughput and the constant risk of a warming event during loading. The instrument that caused the temperature problem is left unchanged.
The CF321 addresses this at the source. An integrated compressor maintains the chamber at any set point from −10°C to +40°C continuously, and the pre-cooling cycle reaches 4°C from ambient in ≤5 minutes — so the centrifuge is cold before the first tube loads. The brushless DC motor eliminates the heat-generating friction that ages brushed motors, and the 5-inch touchscreen gives direct access to speed, RCF, temperature, and all nine programmable protocols without menu-diving. The result: every spin is reproducible to the same conditions as the protocol specifies, regardless of ambient temperature or run duration.
Precision Refrigeration — −10°C to +40°C Continuous
Temperature-controlled centrifugation from −10°C to +40°C with active cooling maintained throughout the run. The chamber reaches 4°C from room temperature in ≤5 minutes — not 20. A built-in condensate drain tank prevents moisture accumulation without requiring manual emptying after every cold run.
21,300 × g at 15,000 RPM — Full Research-Grade Force
The CF321 reaches 21,300 × g at 15,000 RPM — the centrifugal force required for tight pellets in nucleic acid precipitation, protein aggregation assays, and subcellular fractionation. Speed input accepts RPM or RCF directly, and the 16-second acceleration / 15-second deceleration cycle minimizes protocol dead time at maximum speed.
Four Compatible Rotors — One Centrifuge Covers Every Format
Ships with the 24 × 1.5/2.0 mL aerosol-tight rotor. Three additional rotors cover 18 × 1.5/2.0 mL spin columns, 12 × 5 mL tubes, and 4 × 8 × 0.2 mL PCR strips (32 PCR tubes total) — eliminating the secondary centrifuge that most molecular biology labs maintain just for spin-column and PCR-strip work.
5-Inch Touchscreen · 9 Stored Programs · Motorized Lid Lock
The high-resolution 5-inch touch display provides direct entry for all parameters and a status-indicator light bar that changes color for running, warning, and alarm states — readable from across the bench. Nine user-programmable protocols store complex multi-step runs. The motorized double-lock lid closes with light pressure and releases automatically at run completion.
Pre-cool to 4°C in ≤5 minutes — then hold that temperature through 15,000 RPM and back to zero. The CF321's compressor-based cooling system doesn't just bring the chamber down — it actively maintains set temperature under full centrifugal load, protecting RNA, labile kinases, membrane vesicles, and any other sample that fails at room temperature the moment your back is turned.
Key Features
- Speed range: 300–15,000 RPM, 100 RPM increments
- Maximum RCF: 21,300 × g at 15,000 RPM
- Temperature range: −10°C to +40°C continuous
- Pre-cool time: ≤5 minutes from RT (~21°C) to 4°C
- Maximum speed temperature hold: 4°C continuous
- 5-inch high-resolution touchscreen with wide viewing angle
- Color-coded status indicator light bar — run / warning / alarm
- 9 user-programmable protocols for fast method recall
- Speed input in RPM or RCF — direct entry, no conversion
- Acceleration: 16 seconds to max speed
- Deceleration: 15 seconds from max speed
- SOFT brake function for fragile sample pellets
- Run time: 30 seconds to 99 hours 59 minutes / continuous
- Standard rotor: 24 × 1.5/2.0 mL with aerosol-tight biosafety lid
- Optional: 18 × 1.5/2.0 mL spin column rotor
- Optional: 12 × 5 mL rotor
- Optional: 4 × 8 × 0.2 mL PCR strip/tube rotor (32 tubes)
- Motorized double-lock lid — light-pressure closure, auto-release
- Brushless DC motor — maintenance-free, silent, long service life
- Overspeed protection with automatic shutdown
- Imbalance detection and automatic door interlock
- Built-in condensate tank — no manual draining after cold runs
- Noise level: <60 dB(A)
- Universal voltage: AC 220–230 V, 50–60 Hz
Cooling Performance Metrics
≤5 min
Pre-cool to 4°C
From room temperature (~21°C) to 4°C — ready before the first tube loads
−10°C
Minimum Temperature
Sub-zero centrifugation for cryo-sensitive extractions and freeze-thaw sensitive samples
4°C Hold
At Full Speed
Active temperature maintenance at 15,000 RPM — not just at rest, throughout the entire run
Rotor Compatibility
| Rotor | Capacity | Primary Application | Included |
|---|---|---|---|
| 24 × 1.5/2.0 mL | 24 standard microtubes | DNA/RNA extraction, protein pelleting, general molecular biology | Included |
| 18 × 1.5/2.0 mL Spin Column | 18 spin column tubes | Column-based nucleic acid purification, size-exclusion, desalting | Optional |
| 12 × 5 mL | 12 × 5 mL conical tubes | Larger volume pellets, cell harvesting, precipitation assays | Optional |
| 4 × 8 × 0.2 mL PCR Strips | 32 × 0.2 mL PCR tubes | PCR strip centrifugation, library prep, thermocycler pre-spin | Optional |
Ideal Applications
Molecular Biology & Genomics
Nucleic acid extraction, RNA isolation, NGS library preparation, PCR strip centrifugation, and any protocol where temperature excursion between spins introduces downstream variability.
Protein & Biochemistry Research
Labile enzyme isolation, membrane fraction preparation, antibody precipitation, and subcellular fractionation workflows where protein integrity depends on maintaining cold chain through the spin.
Cell Biology & Clinical Research
Suspension cell harvesting, primary cell isolation, exosome preparation, and clinical sample processing — where the quality of the separated pellet or supernatant directly determines assay sensitivity.
What's in the Box
Technical Specifications
| Parameter | Specification |
|---|---|
| Model | CF321 |
| Speed Range | 300–15,000 RPM (increment: 100 RPM) |
| Maximum RCF | 21,300 × g at 15,000 RPM |
| Speed Input Mode | RPM or RCF (direct entry) |
| Acceleration Time | 16 seconds to maximum speed |
| Deceleration Time | 15 seconds from maximum speed |
| SOFT Brake Function | Yes — gentle deceleration for fragile pellets |
| Run Time | 30 seconds to 99 hours 59 minutes / Continuous |
| Temperature Control Range | −10°C to +40°C |
| Maximum Speed Temperature Hold | 4°C continuous at full speed |
| Pre-cooling Time | ≤5 minutes from RT (~21°C) to 4°C |
| Condensate Management | Built-in condensate drain tank |
| Standard Rotor | 24 × 1.5/2.0 mL with aerosol-tight biosafety lid |
| Optional Rotors | 18 × 1.5/2.0 mL spin column · 12 × 5 mL · 4 × 8 × 0.2 mL PCR strips |
| Display | 5-inch high-resolution touchscreen with wide viewing angle |
| Stored Programs | 9 user-programmable protocols |
| Status Indicator | Color-coded bar light — running / warning / alarm |
| Lid Mechanism | Motorized double-lock — light-pressure close, auto-release |
| Motor Type | Brushless DC — maintenance-free |
| Safety Devices | Door interlock · Overspeed protection · Imbalance detection · Over-temperature protection |
| Noise Level | <60 dB(A) |
| Input Power | AC 220–230 V, 50–60 Hz |
| Power Consumption | 600 W |
| Dimensions (W × D × H) | 520 × 305 × 285 mm |
| Weight | 30 kg |
| Warranty | 2-year all-inclusive manufacturer's warranty |
Surge Scientific Warranty & Support
Every CF321 ships with a 2-year all-inclusive manufacturer's warranty covering parts and labor. Our instrument application specialists are available for protocol guidance, rotor selection, and post-purchase technical support. Contact us at 855.943.3344 or info@SurgeScientific.com. In stock at our New Jersey warehouse — most orders ship within 1–2 business days.