Surge Scientific · Model SPT-NanoF · Micro Volume Spectrophotometer · 3-in-1: UV-VIS + OD600 + Fluorescence
SPT-NanoF Micro Volume Spectrophotometer
UV-VIS 190–800 nm + OD600 + Built-in Fluorescence Detection · 0.5 pg/µL dsDNA Detection Limit · Xenon Flash Lamp · 7-inch Touchscreen
UV-VIS + OD600 + Fluorescence · 0.5 pg/µL dsDNA · The advanced 3-in-1 model · 190–800 nm Full Spectrum · 0.5 µL Minimum Sample Volume · Auto-Ranging Pathlength · 15,000 ng/µL Maximum dsDNA Detection · 2 ng/µL Minimum Detection · Xenon Flash Lamp (Zero Warm-Up · 10-Year Service Life) · 2048 px CMOS Detector · 1 nm Wavelength Accuracy · ≤3 nm Spectral Resolution · OD600 Cuvette Mode · 7-inch Color Touchscreen · Android Software · Built-in Printer · USB 2.0 Export · 4.1 kg Compact Footprint
The SPT-NanoF is the advanced flagship model in the Surge Scientific micro-volume spectrophotometer family — combining full UV-VIS spectral analysis (190–800 nm), OD600 bacterial density measurement, AND a built-in fluorescence detection module with 0.5 pg/µL dsDNA detection limit in one compact instrument. Three measurement modes in one footprint: pedestal UV-VIS for routine DNA / protein quantification, cuvette OD600 for bacterial culture density, and fluorescence quantification for the femtogram-class samples (NGS libraries, ctDNA, cfDNA, low-yield extractions) where UV-VIS contamination would inflate the reading. Excitation 435–485 nm / Emission 530–560 nm filters cover standard fluorescence kits for dsDNA, RNA, and protein quantification. R²≥0.995 linear dynamic range with ≤1.5% repeatability. Xenon flash lamp with zero warm-up and 10-year service life. 7-inch color touchscreen running Android software, built-in printer, USB 2.0 export.
Why the SPT-NanoF Fluorescence Module Earns the Price Premium
Modern molecular biology labs typically need both UV-VIS and fluorescence quantification — and most buy them as separate instruments at separate price points consuming separate bench space. UV-VIS is fast, cheap per sample, and works well for high-concentration samples where minor contamination doesn't materially affect readings. Fluorescence is roughly 1000× more sensitive and analyte-specific, but requires reagent kits and slightly slower per-sample reads. Most molecular biology workflows need UV-VIS for routine bulk samples and fluorescence for low-concentration high-stakes QC like NGS library preparation, where a 5% over-quantification due to UV-VIS contamination can mean a failed sequencing run.
The SPT-NanoF eliminates the need for two instruments. Switch modes on the touchscreen — UV-VIS for the routine extract on the pedestal (260 nm with purity ratios 260/280 and 260/230), fluorescence for the precious NGS library that needs 0.5 pg/µL detection in the cuvette slot (with the included PCR Tube Holder for 0.5 mL PCR tubes), OD600 for the bacterial culture growing in the incubator. One pedestal, one Xenon lamp, one touchscreen, one USB export, one bench footprint. The 0.5 pg/µL dsDNA fluorescence sensitivity is competitive with dedicated fluorometers like the Qubit family — which means you don't compromise on quantification quality to get the bench-space and workflow-consolidation advantages. For labs running NGS, ctDNA, cfDNA, or any low-abundance nucleic acid work, the SPT-NanoF is the right purchase from day one.
3-in-1: UV-VIS + OD600 + Fluorescence
Three detection modes in one instrument — UV-VIS spectrophotometry, OD600 bacterial density, AND fluorescence quantification. Replaces 2–3 separate instruments with one bench footprint.
0.5 pg/µL dsDNA Fluorescence
Femtogram-class sensitivity — competitive with dedicated fluorometers. Detection limit 0.5 pg/µL dsDNA. Excitation 435–485 nm / Emission 530–560 nm. R²≥0.995 linear range.
Full UV-VIS 190–800 nm
Same 190–800 nm spectral range as the standard SPT-Nano. 0.5 µL minimum, auto-ranging pathlength, 15,000 ng/µL maximum dsDNA without dilution. Full feature parity for routine UV-VIS work.
7-inch Touchscreen · Android
Mode-switch on touchscreen — UV-VIS / OD600 / fluorescence with one tap. Built-in printer, USB 2.0 export. AC 100-240V universal power supply.
"UV-VIS for the bulk extract. 0.5 pg/µL fluorescence for the NGS library. OD600 for the bacterial culture. One pedestal, one screen, one bill."
Key Features
- Micro-volume UV-VIS spectrophotometer
- Full spectral range 190–800 nm
- 0.5 µL minimum sample volume
- Auto-ranging pathlength (0.05 / 0.2 / 1 mm)
- 15,000 ng/µL maximum dsDNA detection
- 2 ng/µL minimum dsDNA detection
- 0.06 mg/mL minimum BSA detection
- 440 mg/mL maximum BSA detection
- No dilution required for high concentrations
- Pedestal sample retention
- Aluminum alloy + quartz fiber pedestal
- No cuvettes required for routine UV-VIS
- Xenon flash lamp light source
- Zero warm-up time
- 10-year lamp service life
- 2048 px CMOS detector
- 1 nm wavelength accuracy
- ≤3 nm spectral resolution
- 0.003 Abs precision
- 0.04–300 A absorbance range
- Built-in OD600 measurement mode
- OD600 cuvette compartment (12.5×12.5×45 mm)
- OD600 LED light source at 600±8 nm
- Built-in fluorescence detection module
- 0.5 pg/µL dsDNA fluorescence detection limit
- Excitation 435–485 nm
- Emission 530–560 nm
- R²≥0.995 linear dynamic range
- ≤1.5% fluorescence repeatability
- 0.5 mL PCR tube fluorescence sample holder
- PCR Tube Holder for Cuvette Slot included
- Compatible with fluorescence quantification kits
- 7-inch color LCD touchscreen
- Android-based software
- Standalone operation — no PC required
- Intuitive user interface
- Built-in printer
- USB 2.0 data export
- Universal AC 100–240V power supply
- 50/60 Hz operation
- 48W operating power · 8W standby
- Compact footprint (318 × 220 × 205 mm)
- Lightweight at 4.1 kg
- Suitable for molecular biology / clinical / microbiology / chemistry / pharma R&D
- 2-year all-inclusive warranty
Ideal Applications
NGS Library Quantification
Next-generation sequencing library QC where the 0.5 pg/µL dsDNA fluorescence detection delivers Qubit-class accuracy on the same instrument as routine UV-VIS — eliminates the bench-space and workflow overhead of running a separate fluorometer.
ctDNA & cfDNA Analysis
Circulating tumor DNA and cell-free DNA quantification where UV-VIS contamination from co-purified protein / RNA would inflate readings — fluorescence mode delivers analyte-specific quantification at femtogram-class sensitivity.
Multi-Modal Research Cores
Multi-application research cores running diverse molecular biology workflows — UV-VIS for routine extracts, fluorescence for high-stakes low-abundance samples, OD600 for cultures — all on one calibrated instrument with cross-instrument-free measurement consistency.
Model Selection Guide
The Surge Scientific micro-volume spectrophotometer family offers two configurations. Best for: labs running NGS / ctDNA / cfDNA / low-abundance nucleic acid quantification. Pick this variant when your workflow includes femtogram-class samples and the 0.5 pg/µL fluorescence detection delivers Qubit-class accuracy. The standard SPT-Nano is the right choice for labs without fluorescence requirements.
What's in the Box
The SPT-NanoF ships ready for routine use — Xenon lamp, touchscreen, printer, USB all integrated.
Technical Specifications
| Parameter | Specification |
|---|---|
| Model / SKU | SPT-NanoF |
| Type | Micro-volume UV-VIS spectrophotometer with fluorescence detection module |
| Optical & Detection — Shared Across Both Models | |
| Wavelength Range | 190 – 800 nm |
| Minimum Sample Volume | 0.5 µL |
| Path Length (Auto-Ranging) | 0.05 mm (high concentration) · 0.2 mm (high concentration) · 1 mm (normal concentration) |
| Light Source | Xenon flash lamp — zero warm-up · 10-year service life |
| Detector Type | 2048 px CMOS |
| Wavelength Accuracy | 1 nm |
| Spectral Resolution | ≤ 3 nm |
| Absorbance Precision | 0.003 Abs |
| Absorbance Accuracy | 1% (4.096 A at 260 nm) |
| Absorbance Range | 0.04 – 300 A |
| Lower Limit of Detection | 2 ng/µL dsDNA · 0.06 mg/mL BSA |
| Maximum Concentration | 15,000 ng/µL dsDNA · 440 mg/mL BSA |
| Sample Pedestal | Aluminum alloy and quartz fiber |
| OD600 Bacterial Density Measurement | |
| Sample Holder | Cuvette |
| Cuvette Dimensions | 12.5 × 12.5 × 45 mm · Z-Height 8.5 mm |
| OD600 Light Source | LED |
| OD600 Wavelength | 600 ± 8 nm |
| OD600 Absorbance Range | 0 – 4 A |
| Fluorescence Detection — SPT-NanoF Only | |
| Sample Holder | 0.5 mL PCR Tube (with PCR Tube Holder for Cuvette Slot) |
| Excitation Filters | 435 – 485 nm |
| Emission Filters | 530 – 560 nm |
| Limit of Detection | 0.5 pg/µL dsDNA |
| Linear Dynamic Range | R² ≥ 0.995 |
| Repeatability | ≤ 1.5% |
| Power, Software, Connectivity | |
| Power Supply | AC 100–240V · 50/60 Hz (universal power adapter) |
| Operating Power | 48 W |
| Standby Power | 8 W |
| Software | Android-based |
| Display | 7-inch color LCD touchscreen |
| Standalone Operation | Yes — no PC required |
| Data Output | USB 2.0 · Built-in printer |
| Dimensions & Weight | |
| Dimensions (W × D × H) | 318 × 220 × 205 mm |
| Weight | 4.1 kg |
| Companion Variants | Also available as SPT-Nano (without fluorescence) |
| Warranty | 2-year all-inclusive manufacturer warranty |
Surge Scientific Warranty & Support
This instrument is sold through Surge Scientific, your trusted laboratory equipment partner. Our product specialists are available Monday–Friday for assistance. The SPT-NanoF is backed by a 2-year all-inclusive manufacturer's warranty. Contact us at info@surgescientific.com or reach out directly to our support team at 855.943.3344 for pre-sales questions, micro-volume measurement protocols, OD600 calibration, fluorescence kit selection, fluorescence calibration, pedestal maintenance, and technical assistance.